HeLa sh‐IFITM3 were infected with ZIKV HD 78 (MOI 1) for 24 h in the presence or absence of the pan PI3K inhibitor Wortmannin, the class 1‐specific PI3K inhibitor ZSTK474 (1 μM), the Akt inhibitor Triciribine (Akt V) (20 μM), the class 3‐specific PI3K (vps34) inhibitor SAR405 (1 μM), or the pan‐PI3K inhibitor 3MA (5 mM). Movie EV3 is a representative example of data used for quantification. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Here, we studied the morphological cellular changes induced by ZIKV and addressed the role of interferon-induced transmembrane proteins (IFITM), a family of broad-spectrum antiviral factors, during viral replication. Brefeldin A (0.5 μg/ml) was added during the 24 h of infection. Zika Virus: a re-emerging pathogen with rapidly evolving public health implications. (B) The percentage of E+ cells was quantified with 4G2 staining and flow cytometry. The black triangle indicates increasing amounts of virus (MOI 3 and 10). ZIKV ‐induced cytoplasmic vacuolization and cytopathic effects, Time‐lapse analysis of the morphological changes…. eCollection 2018 Jun. HeLa sh‐IFITM3 were infected with ZIKV HD78 or DV2 for 24 h or 5 days pi. Draz MS , Venkataramani M , Lakshminarayanan H , Saygili E , Moazeni M , Vasan A , Li Y , Sun X , Hua S , Yu XG , Shafiee H . Mean ± SEM from three independent experiments is shown. ZIKV E expression determined by flow cytometry and the proportion of cells displaying vacuoles was scored by visual examination of at least 200 cells at 24 h pi in HeLa sh‐SCR or sh‐IFITM3 cells infected with the indicated MOIs. The Zika virus can infect and alter cells in the human nervous system that are crucial for formation of bones and cartilage in the skull, a study has revealed. 8 comments 1 video (C) In parallel, the proportion of cells displaying vacuoles was scored by visual examination of at least 200 cells. We report that ZIKV induces massive vacuolization followed by "implosive" cell death in human epithelial cells, primary skin fibroblasts and astrocytes, a phenomenon which is exacerbated when IFITM3 levels are low. Please enable it to take advantage of the complete set of features! Results are mean ± SEM for three independent experiments. When infected during gestation, fetuses have their immature neural cells killed by the virus and consequently have devastating findings at birth. (E) The percentage of E+ cells was quantified with 4G2 staining and flow cytometry (left panel).
Large cytoplasmic vacuoles are the direct result of productive ZIKV infection. Toll-like receptor 3 regulates Zika virus infection and associated host inflammatory response in primary human astrocytes. Still images were extracted from Movie EV5 at the indicated time points. Saving Lives, Protecting People, Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Zika Vector Control in the Continental US, Healthcare Exposure to Zika & Infection Control, Interim Guidance for Managing Occupational Exposures to Zika Virus for Healthcare Personnel, U.S. Department of Health & Human Services. The mean fluorescence intensity (MFI) of 4G2 staining was determined by flow cytometry. Cell Microbiol 17: 45–50
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